The correlativity analysis of six methods of detecting apoptosis

摘要

The aim of this study was to compare six methods of detecting apoptosis induced by extracellular adenosine triphosphate(ATP)in human leukemic lymphocytes with purinergic P2Z receptors. These meth- ods used were electron microscopy(EM), detection of internucleosomal DNA fragmentation by agarose gel electrophoresis, autoradiographic analysis of DNA fragmentation, in situ labeling of DNA strand breaks with fluroescein-dUTP and exogenous terminal deoxynucleotidyl transferase(TUNEL), quantitation of 3'-ends of DNA breaks by labeling withα~32PdCTP(TdT assay), and quantitation of apoptotic cells with fluorescein-annexin V using flow cytometry(FCA).