Overexpression of bcl-2 protects hepatoma cell line HCC-9204 from ethanol-induced apoptosis

摘要

Conclusion Overexpression of Bcl-2 protein suppresses ethanol-induced apoptosis of the HCC cell line HCC-9204. Objective To investigate the effect of overexpression of bcl-2 on ethanol-induced apoptosis of primary Hepatocellular carcinoma(HCC)cells. Methods The retrovirus expression vector pDOR-SB containing human bcl-2 cDNA was introduced into a Human HCC cell line HCC-9204 by liposome-mediated transfection. PDOR-transfected and non-transfected HCC-9204 cells were used as controls. The expression of Bcl-2 protein by transfected HCC-9204 cells was Detected by the immunohistochemical method.