Method for Increasing Humanized Antibody Production in CHO Cells by Overexpression of bcl-2 Gene and Treatment of Sodium Butyrate

摘要

PURPOSE: Provided is a method for increasing humanized antibody production in CHO cells by overexpression of bcl-2 gene and treatment of sodium butyrate, thereby increasing unit production of animal cell products, such as humanized antibodies and cytokines. CONSTITUTION: The antibody production is increased by introducing a vector which overexpresses bcl-2 gene into CHO cells transformed with a gene encoding a humanized antibody to obtain a cell line having apoptosis resistance, then adding sodium butyrate. The method for increasing the production of humanized antibodies comprises the steps of: introducing a vector which overexpressing human bcl-1 gene into the recombinant CHO cells above; inoculating the recombinant CHO cells transformed with the vector to a medium; and adding sodium butyrate thereto.