The Correlation of p53 and nm23-H1 Expression with Invasiveness and Metastasis in Esophageal Carcinoma

摘要

Objective To study the relationship between expression of p53 and nm23-H1 and differentiation, invasiveness and metastasis in human esophageal carcinoma, and the correlation between expression of p53 and nm23-H1. Methods Expression of p53 and nm23-H1 in 50 patients with squamous cell carcinoma of esophagus was detected by using immuno-histochemical S-P methods. Results 35 cases (70% ) and 32 cases (64% ) of esophageal squamous cell carcinoma were positive for nm23-H1 protein and p53 protein, respectively. The expression of nm23-H1 was related to lymphatic metastasis (P <0.025) , but not related to tumor differentiation, invasiveness, tumor location, tumor length, patient' s gender and age(P >0.05) . The lymphatic metastasis location positive group had a very lower expression of nm23-H1 and the negative rate was 70. 8% , but the negative group had a higher expression and the positive rate was 65.4%. The expression of p53 was related to tumor differentiation and invasiveness(P<0.05) , but not related to lymphatic metastasis, tumor location, tumor length, patient's gender and age(P>0. 05). Among the three groups, the high differentiation group had the lowest expression of p53 and the positive rate was 29. 2% , but the low differentiation group had the highest positive rate (71.4%). As for tumor invasiveness, the group of outer membrane of esophagus infiltrated had the highest p53 protein positive rate (56%) , but in the group of mucous or submucous layer infiltrated p53 protein was not detectable. The low expression of nm23-H1 and the high expression of p53 were also correlated. The expression of nm23-H1 and p53 were both correlated with TNM stage of esophageal carcinoma ( P <0. 05). The better esophageal carcinomas differentiated, the lower nm23-H1 expressed and higher p53 expressed. Conclusion Low expression of nm23-H1 and high expression of p53 play an important role in the progression of squamous cell carcinoma of esophagus. Mm23-H1 might be a gene marker in the prophecy of patients' prognosis and benefit tumor treatment clinically.