Analysis of amino acid constituents of gallstones

摘要

AIM To seek effective drugs to dissolve bilirubin, glycoprotein and black stones and better lithotriptics for cholesterol stones, and study the amino acid constituents of gallstones. METHODS According to the characteristics of infrared spectrum and the contents of bilirubin tested by semiquantitative chemical analysis, 30 of 148 cases of gallstones were selected and divided into 5 groups. Amino acids of the 30 cases were detected by high speed chromatography. RESULTS The quantity of amino acids in black stones was the highest (226.9 mg/g) and that of pure cholesterol stones was the lowest, only 1.4 mg/g in a case. In five groups of gallstones, the contents of amino acids were: black stone > mixed bilirubin stone and glucoprotein stone > mixed cholesterol stone > pure cholesterol stone. The proportions were: 95.95: 29.02 and 28.05:5.78: 1. Aliphatic amino acids accounted for about 50% of the total amino acids in gallstones and glycine 15.3% of the total amount of 17 kinds of amino acids. CONCLUSION In mixed stones, the higher level of bilirubin, the higher contents of amino acids. The acidic amino acids of bilirubin stones were relatively higher than those of cholesterol stones.