Production manner of the amino acid fluorescent derivative blend for amino acid analysis and the amino acid fluorescent derivative blend for amino acid analysis and uses the amino acid fluorescent derivative blend for amino acid analysis the manner

摘要

A new mixture of fluorescent amino acid derivatives suitable for use in quantitative amino acid analysis is provided in which all primary amino acids are present in one kind of derivatization whereas all secondary amino acids are present in another kind of derivatization. …??The process for recovering the mixture comprises the steps of derivatization of the primary amino acids of an amino acid mixture with ortho-phthalaldehyde (OPA) and of derivatization of the secondary amino acids with fluorenylmethylchloroformate (FMOC) in the presence of acetonitrile. The mixture can be obtained automatically and is highly suitable for fast analysis by reversed-phase liquid chromatography.