Isolation and Characterization of Proteins Interacting with Activin Type Ⅱ Receptors

摘要

Regulation of the number of activin receptors that are present in the cell membrane plays a key role in the modulation of cellular responses to activin. In order to find the regulators, a novel protein ARIPzip, interacting with activin type Ⅱ receptors, was searched and identified by using yeast two-hybrid screening. ARIPzip is a splicing variant of ARIP2. This has been discussed previously. ARIPzip can specifically interact with ActR ⅡA, and is widely distributed in mouse tissues. Overexpression of ARIPzip can cause the activin-induced transcriptional activities to increase in a dose-dependent manner while the overexpression of ARIP2 can decrease these activities. These data suggest that the C-terminal regions of ARIP2 and ARIPzip are involved in the regulation of activin signaling.