DNAzyme catalytic beacon sensors that resist temperature-dependent variations

摘要

The temperature-dependent variability of a Pb~(2+)-specific 8-17E DNAzyme catalytic beacon sensor has been addressed through the introduction of mismatches in the DNAzyme, and the resulting sensors resist temperature-dependent variations from 4 to 30 ℃.rnThe demand for simple, on-site, real-time detection and quantification of heavy metal ions is increasing every 'day, since metal ions such as lead and mercury are common environmental contaminants that have significant adverse affects on human health.