Comparing dendritic with linear esterase peptides by screening SPOT arrays for catalysis

摘要

Fluorescence screening of a 96-membered SPOT library of histidine containing dendritic and linear peptides revealed the remarkable esterolytic activity of short histidine oligomers that show catalytic proficiencies within one order of magnitude of histidine-containing esterase peptide dendrimers. Enzyme catalysis arises by cooperativity between amino acid side chains upon folding of the polypeptide, which enhances the catalytic power of functional groups and enables substrate binding by formation of a macromolecular receptor.