Chemoenzymatic synthesis of glycosylphosphatidylinositol-anchored glycopeptides

摘要

MUC1 glycopeptide was efficiently coupled to glycosylphospha-tidylinositol (GPI) derivatives by sortase A (SrtA), verifying that SrtA can accept sterically hindered glycopeptide as substrate for ligation with GPIs. This work has established a practical method for the chemoenzymatic synthesis of GPI-linked glycopeptides.