Regenerable electrochemical immunological sensing at DNA nanostructure-decorated gold surfaces

摘要

A regenerable electrochemical immunosensor with novel 3D DNA nanostructure-decorated gold surfaces was developed by taking advantage of DNA-directed antibody conjugation and high resistance to non-specific protein adsorption. DNA, an essential molecule in genetics, has attracted rapidly increasing attention as a kind of nature-endowed functional nanomaterial. DNA molecules possess unparalleled self-recognition properties, offering great flexibility and convenience to "bottom-up" construct exquisite nanostructures with extraordinarily high controllability and precision.' Consequently, DNA nanotechnology has been actively explored for a wide range of applications, such as nanofabrication and molecular electronics, biosensing and drug delivery.2 Since the pioneering work of Seeman in the early 1980s, artificial DNA nanostructures of various sizes, shapes and dimensions have been designed and constructed. More recently, rapid advances have been seen in the design and applications of three-dimensional (3D) DNA architectures.3 Particularly, the Turberfield group