Encoding electrochemiluminescence using Ru(bpy)3~(2+) and fluorescein isothiocyanate co-doped silica nanoparticles

摘要

In this paper, an electrochemiluminescence (ECL) encoding method was developed for the first time, based on a dual-dye system including Ru(bpy)_3~(2+) as an ECL emitter and fluorescein isothiocyanate (FITC) as a coding dye. During the past five decades, most electrochemiluminescence (ECL) studies were based on tris(2,2'-bipyridyl)ruthenium (Ru(bpy)3 ), due to its solubility in both aqueous and non-aqueous solvents and, especially, its high ECL efficiency in aqueous solution compared to all the other reported ECL reagents. Although the excellent characteristics of Ru(bpy)_3~(2+) result in it being widely applied, the weakness of the Ru(bpy)_3~(2+) system is obviously that its single wavelength makes the analytical capacity of this system very limited.