First synthetic analogues of diphosphoinositol polyphosphates: interaction with PP-InsP_5 kinase

摘要

We synthesised analogues of diphosphoinositol polyphosphates (PP-InsPs) in which the diphosphate is replaced by an α-phos-phonoacetic acid (PA) ester. Structural analysis revealed that 5-PA-InsP_5 mimics 5-PP-InsP_5 binding to the kinase domain of PPIP5K2; both molecules were phosphorylated by the enzyme. PA-InsPs are promising candidates for further studies into the biology of PP-InsPs.