Alteration of enzyme activity and enantioselectivity by biomimetic encapsulation in silica particles

摘要

Direct encapsulation of esterase or lipase fused with the silica-precipitating R5 peptide from Cylindrotheca fusiformis in silica particles afforded high yields of active entrapped protein. The hydrolytic activity of both enzymes against p-nitrophenyl buty-rate was similarly affected by encapsulation and the enantioselectivity of the esterase was both improved and inverted.