Resolving DNA at efficiencies of more than a million plates per meter using bare narrow open capillaries without sieving matrices

摘要

We report a novel approach for effectively separating DNA molecules in free solution. The method uses a bare narrow open capillary without any sieving matrices to resolve a wide size-range of DNA fragments at efficiencies of more than a million plates per meter routinely. Genes are DNA segments that cany the genetic instructions used in developing all living organisms. There are about 20 000-25 000 such genes in the human body. Separating and analyzing DNA fragments are essential to understanding and modulating how DNA works. DNA separations are usually carried out by slab-gel electrophoresis, while pulsed-field gel electrophoresis (PFGE) is employed particularly for resolving large DNA fragments.