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首页> 外文期刊>Chemical Communications >Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease
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Highly sensitive methyltransferase activity assay and inhibitor screening based on fluorescence quenching of graphene oxide integrated with the site-specific cleavage of restriction endonuclease

机译：基于氧化石墨烯的荧光猝灭与限制性内切酶的位点特异性切割相结合的高灵敏甲基转移酶活性测定和抑制剂筛选

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摘要

We report a sensitive and selective approach for the DNA methyl-transferase (MTase) activity assay and MTase inhibitor screening by coupling the fluorescence quenching of graphene oxide with site-specific cleavage of a restriction endonuclease.
机译：我们报告了DNA甲基转移酶（MTase）活性测定和MTase抑制剂筛选的灵敏和选择性的方法，方法是将氧化石墨烯的荧光猝灭与限制性内切核酸酶的位点特异性裂解相结合。

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  • 来源
    《Chemical Communications》 |2014年第73期|10691-10694|共4页
  • 作者

    Lijuan Ji; Zhewei Cai; Yingdan Qian; Ping Wu; Hui Zhang; Chenxin Cai;

  • 作者单位

    Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China;

    Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China;

    Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China;

    Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China;

    Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China;

    Jiangsu Key Laboratory of New Power Batteries, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China;

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