Ultrasensitive detection of thrombin using surface plasmon resonance and quartz crystal microbalance sensors by aptamer-based rolling circle amplification and nanoparticle signal enhancement

摘要

The surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) aptasensors combined with rolling circle amplification and bio-bar-coded AuNP enhancement have been applied to detect the human α-thrombin for the first time. The assay platform exhibited excellent selectivity and sensitivity with detection limit as low as 0.78 aM.