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Effects of emodin and double blood supplies on liver regeneration of reduced size graft liver in rat model

机译:大黄素和双重血液供应对大鼠减径移植肝肝脏再生的影响

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AIM: To study the influences of emodin and reconstruction of double blood supplies on liver regeneration of reduced size graft liver in rat model. METHODS: A total of 45 SD-SD rat reduced size liver transplantation models were randomly divided into three groups (A-C). The conventional reduced size liver transplantation was performed on rats in group A, while the hepatic artery blood supply was restored in groups B and C. The emodin (1.5 mg/kg/d) was given by intraperitoneal route in group C only. The recipients were killed on the seventh day after the operation. The proliferative cell nuclear antigen (PCNA), TBil and ALT of serum were detected, and the pathological changes of liver cell were observed. RESULTS: The numbers of the rats that survived in A, B, and C group on the seventh day after operation were 14, 13,13, respectively. The levels of TBil (31.5±5.2 μmol/L, 23.2±3.1 μmol/L vs 38.6±6.8 μmol/L), and ALT (5 351± 1 050 nKat, 1300±900 nKat vs 5779±1202 nKat) in serum in groups B and C were lower than those in group A (P < 0.05), while the expression of PCNA in groups B or C was higher than that in group A (22.0±3.5%, 28.2±4.2% vs 18.6±3.2%, P < 0.05). The deeper staining nuclei, double nuclei, multi-nuclei and much glycogen were observed in liver cells of groups B and C, especially in group C, while fewer were found in liver cells of group A. CONCLUSION: The reconstruction of arterial blood supply is very important for rat liver regeneration after reduced size liver transplantation. Emodin has the effect of promoting liver regeneration and improving liver function in rats after reduced size transplantation. The possible mechanism is improving proliferation of liver cell and protecting liver cells from injury.
机译:目的:研究大黄素和双血供重建对大鼠小尺寸移植肝肝脏再生的影响。方法:将45只SD-SD大鼠缩小尺寸的肝移植模型随机分为三组(A-C)。 A组在大鼠上进行了常规尺寸缩小的肝移植,而B组和C组则恢复了肝动脉血供。大黄素(1.5 mg / kg / d)仅在C组中通过腹膜内途径给药。接受者在手术后的第七天被杀死。检测血清中增殖细胞核抗原(PCNA),TBil和ALT,观察肝细胞病理变化。结果:术后第7天,A,B和C组存活的大鼠分别为14、13、13。血清中TBil(31.5±5.2μmol/ L,23.2±3.1μmol/ L vs 38.6±6.8μmol/ L)和ALT(ALT 5351±1050 nKat,1300±900 nKat vs 5779±1202 nKat) B和C组均低于A组(P <0.05),而B或C组中PCNA的表达高于A组(22.0±3.5%,28.2±4.2%vs 18.6±3.2%, P <0.05)。 B和C组,尤其是C组的肝细胞中观察到较深的染色核,双核,多核和大量糖原。结论:A组的肝细胞中染色核少。缩小肝脏移植后的大鼠肝脏再生非常重要。大黄素具有减少大小的移植后促进大鼠肝脏再生和改善肝功能的作用。可能的机制是改善肝细胞的增殖并保护肝细胞免受损伤。

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