Expression of cyclic GMP-inhibited phosphodiesterases 3A and 3B (PDE3A and pDE3B) in rat tissues: differential subcellular localization and regulated expression by cyclic AMP

摘要

A combination of pharmacological, molecular biological and biochemical approaches were used to investigate the differential expression of two cyclic GMP-inhibited cyclic nucleotide phosphodiesterase genes (PDE3A and PDE3B) in the rat. RT-PCR using PDE3A- or PDE3B-specific oligonucleotide primers allowed amplification of Products encoding PDE3A(508 bp) or PDE3B(499 bp) sequences from several rat tissues (heart, aorta, Liver, kidney and epididymal fat), from primary cultures of aortic vascular smooth muscle cells (VSMC) As well as from an SV40 large T-antigen immortalized aortic VSMC line.