Dual coupling of heterologously-expressed rat P2Y_6 nucleotide receptors to N-type Ca~2+ and M-type K~+ currents in rat sympathetic neurones

摘要

1. The P2Y_6 receptor is a uridine nucleotide-specific G protein-linked receptor previouslyreported to stimulate the phosphoinositide (PI) pathway. We have investigated its effect in neurones, by micro-injecting its cRNA into dissociated rat sympathetic neurones and recording respnses of N- type Ca~+ (I_Ca(N)) and M-type K~+ (I_k(M)) currents. 2. In P2Y_6 cRNA-injected neurones, UDP or UTP produced a voltage-dependent inhibition of I_Ca(N)) by ～ 53/100 in whole-cell (disrupted-patch) mode and by ～ 73/100 in perforated-patch mode; no Inhibition occurred in control cells.