Arachidonic acid prevents fatty liver induced by conjugated linoleic acid in mice

摘要

Conjugated linoleic acid (CLA) has anti-obesity effects, but induces fatty liver in mice. The present study investigated whether co-administrationnof arachidonic acid (ARA) attenuates fat accumulation in the mouse liver induced by CLA. Male mice (8 weeks old) were given diets with eithernno addition of dietary fat (control), 3% linoleic acid (LA), 3% CLA, 3% CLA þ 1% ARA, or 3% CLA þ 2% ARA for 4 weeks. The perirenalnfat weight in ARA-treated groups decreased similarly as with CLA alone, when compared to control or LA. Plasma TAG concentration was sig-nnificantly higher in the CLA group than in either CLA þ ARA group, while plasma cholesterol and NEFA concentrations did not vary among thengroups. In contrast to visceral fat, liver weight was significantly higher in the CLA group than in the control or LA groups, and the effects of CLAnwere attenuated by ARA. TAG and cholesterol were markedly accumulated in the liver with dietary CLA, whereas co-administration with ARA, atneither concentration, suppressed CLA-induced lipid accumulation. Liver PGE2 was enhanced by a combination of CLA and ARA when comparednwith CLA alone, but PGE1 level was not significantly different among groups. In conclusion, fatty liver induced by CLA was attenuated bynco-administration with ARA, furthermore, a combination of these fatty acids maintained the anti-obese effect of CLA.