Over-expression and characterization of recombinant prefoldin from hyperthermophilic archaeum Pyrococcus furiosus in E. coli

摘要

Prefoldin is a hexameric chaperone that has been identified in eukaryotic cells and in Archaea. E. coli cells over-expressing the prefoldin gene from the hyperthermophilic, archaeum, Pyrococcus furiosus, grew well at 48°C while control cells were unable to grow above 46°C. The isolated and purified Pfu-prefoldin (specially the β subunit and the prefoldin) thermally protected the activity of lysozyme. Keywords Escherichia coli - Prefoldin - Pyrococcus furiosus - Thermo-tolerance