Statistical Analysis And Validation Ofprocess Parameters Influencing Lovastatinrnproduction By Monascus Purpureus Mtccrn369 Under Solid-state Fermentation

摘要

Monascus, a fermented rice (red mold rice), was found to reduce total cholesterol and triglyceride in serum due to the presence of lovastatin, a 3-hydroxy-3-methyl glutaryl coenzyme A (HMG-CoA) reductase inhibitor. Optimization and validation of different process parameters such as temperature, fermentation time, inoculum volume, and pH of the solid medium was done using Box-Behnken's factorial design of response surface method for maximum production of lovastatin by Monascus purpureus MTCC 369. A maximum lovastatin production of 3.422 mg/g was predicted by day 14.43 of fermentation in a rice based solid medium of pH 6 when fermented at a temperature of 29.46℃, an inoculum volume of 5.11 mL, and using response surface plots and the point prediction tool of Design Expert 7.1.3 (Statease Inc., USA) software.