Enhanced Resistance to Bacterial Pathogen in Transgenic Tomato Plants Expressing Cathelicidin Antimicrobial Peptide

摘要

A human cathelicidin antimicrobial peptide (hCAPlS/LL-37) is the only member of the mammalian cathelicidin family of proteins that is present in humans. The LL-37 gene was fused to the secretory signal peptide sequence (sp) and a new construct, pSPl-spLL-37, was transformed into tomato. Integration of single copy of the LL-37 was confirmed by TaqMan-PCR and its expression was confirmed by RT-PCR and ELISA assay. The transgenic tomato plants exhibited significant resistance to bacterial soft rot and bacterial spot where it showed strong concerted expression of PR-protein, LTP and AFP1 genes. In vitro screening of protein extracts isolated from the leaves of transgenic plants delimited the growth of P. carotovorum ssp. carotovorum to 15%, and that of X. campestris pv. vesicatoria to 35%. The subcellular localization of LL-37::GFP fusion protein was mainly localized in the cell membrane and cytoplasm. LL-37 expressing-tomato could therefore provide reliable bio-protection against bacterial soft rot and bacterial spot.