Molecular Genetic and Chemotaxonomic Identification of the Bacterium of the Genus Ochrobactrum Possessing Oil-Oxidizing and Nitrogen-Fixing Activity

摘要

It was shown that analysis of cellular fatty acids using gas chromatography-mass-spectrometry and application of the gene encoding beta-subunit of DNA gyrase (gyrB) as an alternative phylogenetic marker supplemented the taxonomic description of the biotechnologically valuable strain Ochrobactrum sp. IB DT-5.3/2 but were ineffective for species identification of the bacterium. However, based on the results of the matrix-assisted laser desorption-ionization time of flight mass spectrometry of the protein fractions and our previous studies of the cultural-morphological and biochemical features, as well as sequencing of the 16S rRNA gene, we can conclude that the strain Ochrobactrum sp. IB DT-5.3/2 refers to the species O. intermedium.