Structural analysis of compound I in hemoproteins: Study on Proteus mirabilis catalase

摘要

Ferryl catalysis has attracted considerable interest, because a diverse variety of enzymes use ferryl intermediates to perform difficult chemistry. The structure of the reactional intermediate compound I of Proteus mirabilis catalase (PMC) has been solved using time-resolved X-ray diffraction techniques and single crystal microspectrophotometry. Formation of compound I is characterized by signify- cant changes in the absorbance spectrum, and the creation of an oxoferryl group on the distal side of the heme.