Structural and serological studies of the O-antigen of Proteus mirabilis O-9

摘要

The following structure of the O-polysaccharide (O-antigen) of the lipopolysaccharide of Proteus mirabilis O-9 was determined by NMR spectroscopy including 2D ~1H,~1H COSY TOCSY ROESY and ~1H ~13C HMOC experiments along with chemical methods: where the degree of O-acetylations is ~70% Immunochemical studies using rabbit polyclonal anti-Proteus mirabilis O-9 serum showed the importance of O-acetyl groups in manifesting the serological specificity of the O-9 antigen.Anti-P mirabilis O-9 cross-reacted wtih the lipopolysaccharides (LPS) of P vulgaris O-25 and Proteus penneri 14 which could be accounted for by a structural similarity of their O-polysaccharides.