Improved Lactic Acid Production by In Situ Removal of Lactic Acid During Fermentation and a Proposed Scheme for Its Recovery

摘要

Extractive fermentationwith 155.8 g/L initial glucose and 364 g of anion exchange resin yielded a 1.2-fold increase in total lactate produced and a 5.9-fold increase in productivity compared with standard batch fermentation at the same glucose concentration. The addition of resin also served as a pH control strategy. Elution of the resinbound lactate using 1MHCl at 0.1 bed volume/min resulted in complete recovery of lactate. Further extraction of the eluted lactate and lactate in fermentation brothwas conducted with reactive extraction using trioctylamine in octanol. The extraction also provided partial purification of the product, as protein from fermentation broth did not migrate to the extracted solution with the lactate. The recovery of lactate was up to 70 % of the total lactate produced.