Protection of Fenneropenaeus chinensis (Osbeck, 1765) against the white spot syndrome virus using specific chicken egg yolk immunoglobulins by oral delivery

摘要

Two kinds of specific chicken egg yolk immunoglobulins (IgYs), IgY-WSSV and IgY-VP28, were, respectively, raised against the 2 mM binary ethylenimine (BEI)-inactivated white spot syndrome virus (WSSV) and a principal envelope protein VP28. The activity of purified specific IgYs was stable under the conditions of 20–70 °C, pH 3.0–10.0 and 0–700 g L−1 sucrose solution. In the neutralization assay, these high-affinity IgY antibodies can specifically bind with the virus particles to protect shrimp (Fenneropenaeus chinensis) against  WSSV infection. After oral delivery for 20 days, the IgY-WSSV exerted a higher protection effect (RPS: 71.5%) than IgY-VP28 (RPS: 63.7%). Moreover, an increase in RPS (79.2%) was found on addition of IgY-WSSV:VP28 (0.1% IgY-VP28 plus 0.2% IgY-WSSV). This may indicate that neutralization of WSSV refers to the multiple-hit model. By time-course study of the levels of the specific IgYs in vivo, the data showed that the titre was enhanced to a relatively high level (P/N=8.35±0.45) at 3 days post administration, declined slightly (P/N=7.13±1.01) at 7 days post administration and then remained stable for further investigation. The stable antibody level potentially contributes towards blocking a large number of WSSV particles from entering and infecting on the major tissues at the early and late stages after challenge in shrimp.