Detection of lipid bilayer and peptide pore formation at gigahertz frequencies

摘要

A coplanar waveguide defined on a borosilicate glass chip is integrated with a microfluidic cartridge to allow for characterization of lipid bilayer membranes at gigahertz frequencies. The membranes are deposited on the waveguide using a vesicle fusion method. The deposition of the bilayers results in a significant change in the high-frequency transmission properties of the waveguide. We also embed alamethicin, an ion channel forming peptide, in the bilayers. The influence of the pore forming peptides can be clearly detected in the transmission signal.