ドデシル硫酸ナトリウムを用いる底質からの多環芳香族炭化水素類の抽出と定土

摘要

ドデシル硫酸ナいノウム（SDS）によって底質から多環芳香族炭化水素類（Ms）を抽出し，界面活性剤を塩酸によって相分離した後にHPLC一蛍光検出する方法について検討した．抽出の際のSDSの添加量，相分離の際の塩酸濃度，加熱温度，時間等について検討し，最適条件を試料中のSDS濃度を0．1M，塩酸濃度を4M，加熱温度を60℃，濃縮時間を90分間とした．nd止Isの濃縮率は，キニザリングリーンSSを濃縮指標とし，相分離の前後でのキニザリングリーンSSの濃度変化から求めることができた．最適化条件におけるSDS界面活性剤相へのnusの濃蘭率はおよそ10倍，模擬底質からのアントラセン，フルオランチン．ビレンの添加回収率は95％以上を示した．中国東北部吉林省於花江の底質のMs分析に同法を適用したところ，アントラセン，フルオランテン，ビレンの濃度はそれぞれ80帽／kg，230帽／短，220帽／kgとなった．%A determination method for polycyclic aromatic hydrocarbons (PAHs) in sediment samples was investigated on the basis of HPLC with fluolesence detection after the extraction of PAHs from sediment with sodium dodecyl sulfate (SDS), followed by the phase separation of an SDS-rich phase from water with HC1. The concentration of SDS for extraction, the concentration of HC1 and the temperature for phase separation were investigated and optimized. The accumula­tion efficiency of PAHs was estimated by measuring the concentration of Quinizarine Green SS before and after phase separation. In the optimized condition, 0.1 M SDS, 4 M HC1, 60X^ and 90 min for phase separation, the recovery ratio of antracene, fluoranthene and pyrene from a model sediment sample was more than 95% and the accumulation times were about 10. The proposed method was applied to sediment sample collected from the Songhua river in China; the concentrations of antracene, fluoranthene and pyrene in the sediment were 80 Hg/kg, 230 ug/kg and 220 |ig/kg, respectively.