ポリマー化シクロデキストリンとポリエチレンオキシド混合系を新規分離場とするDNAのキャピラリー電気泳動分離

摘要

In this study, we present the first example of the separation of DNA by capillary electrophoresis (CE) using a new sieving matrix, a mixture of the poly-β-cyclodextrin (poly-β-CD) and polyethylene oxide) (PEO). The poly-β-CD and PEO were used as a pseudostationary phase to improve the resolution of DNA fragments. CE separation was performed using a DB-17 coating capillary at an applied voltage of — 10 kV (detector at anode side). Ammonium acetate buffer (25 mmol L~(-1) pH 6.0) was used as a background electrolyte and all sample solutions were prepared by the buffer. The best CE separation was obtained at a concentration of 5 mg mL~(-1) poly-β-CD and 1 mg mL~(-1) PEO. Under these conditions, 13 DNA fragments with sizes ranging from 200 bp to 4000 bp were completely resolved within 12 min, and the peak efficiencies ranged from 91000 to 234000 plates m~(-1). The results presented in this study show the advantage of PEO containing poly-β-CD for DNA separation, including rapidity, high resolving power, and ease of analysis.%近年，キヤピラリー電気泳動法（CE）を用いた生体関連物質の分離が注目を集めている．中でも，DNAやタンパク質の分離に金ナノ粒子（GNPs）と直鎖の高分子化合物，例えばポリエチレンオキシド（PEO）との混合物を擬似固定相とした分離が多数報告されている．一例として，Huangらは，クエン酸修飾GNPsと分子量8MDaのPEOを用いて8～2176bpのDNA分離に成功しており，クエン酸添加量によってGNPsの粒径を変化させた場合の分離傾向について詳細に述べている．