Coomassie Brilliant Dyes as Surface-Enhanced Raman Scattering Probes for Protein−Ligand Recognitions

摘要

Coomassie brilliant dyes have high affinity to proteins andnhigh Raman activity, and on the basis of which, we havenemployed brilliant blue R-250 (BBR) and brilliant bluenG-250 (BBG) as surface-enhanced Raman scatteringn(SERS) labels to probe protein ligand recognitions. Thisnmethod differs from previously proposed methods in thatntarget proteins are labeled rapidly before biological rec-nognitions without procedures of separation and purifica-ntion, rather than attaching Raman labels to metal nano-nparticles, which significantly simplifies the Raman dyenlabeling procedure. In typical assays, ligand-functionalizednmetal nanoparticles assemble by target protein-specificnbindings and this assembly sequentially turns on electro-nmagnetic enhancement of Raman scattering of the pro-nposed labels. The method with its advantages of rapid-nness, high sensitivity, and spectral multiplexing has greatnpotential in probing protein protein and protein smallnmolecule recognitions not only in solution systems butnalso on flexible solid substrates.