Detection of Prostate-Specific Antigen with a Paired Surface Plasma Wave Biosensor

摘要

In this study, we demonstrated that an amplitude-sensi-ntive paired surface plasma wave biosensor (PSPWB) isncapable of real-time detection of prostate-specific antigenn(PSA) in diluted human serum without labeling. Experi-nmentally, the detection limit of PSPWB was 8.4 × 10 9nrefractive index unit (RIU) and the PSPWB couldnmeasure PSA in a phosphate buffered saline solutionnfrom 10 fg/mL (∼300 aM) to 100 pg/mL (∼3 pM)nsuccessfully, with demonstration of a linear relation-nship between PSA concentrations and surface plasmonnresonance (SPR) signals. Therefore, results were ob-ntained over a wide dynamic range 5 orders of magni-ntude for analyte concentration. In addition, the PSPWBnsuccessfully detected PSA in diluted human serum asnwell. These experimental results indicate that thenPSPWB is capable of detection with high sensitivitynover a wide range by using SPR-based biosensors andnhas a capability of detecting biological analytes in clini-ncal sample without complicated operating procedures.