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Detection of Swine-origin Influenza A (H1N1) Viruses Using a Paired Surface Plasma Waves Biosensor

机译:使用配对的表面等离子波生物传感器检测猪源性甲型H1N1流感病毒。

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摘要

In order to enhance the sensitivity of conventional rapid test technique for the detection of swine-origin influenza A (H1N1) viruses (S-OIVs), we used a paired surface plasma waves biosensor (PSPWB) based on SPR in conjunction with an optical heterodyne technique. Experimentally, PSPWB showed a 125-fold improvement at least in the S-OIV detection as compared to conventional enzyme linked immunosorbent assay. Moreover, the detection limit of the PSPWB for the S-OIV detection was enhanced 250-fold in buffer at least in comparison with that of conventional rapid influenza diagnostic test.
机译:为了提高常规快速测试技术检测猪源性甲型H1N1流感病毒(S-OIVs)的敏感性,我们将基于SPR的配对表面等离子体波生物传感器(PSPWB)与光学外差结合使用技术。实验上,与传统的酶联免疫吸附测定法相比,PSPWB至少在S-OIV检测中显示出125倍的改善。此外,至少与常规快速流感诊断测试相比,用于S-OIV检测的PSPWB的检测限至少在缓冲液中提高了250倍。

著录项

  • 来源
    《Biosensing III》|2010年|p.775905.1-775905.6|共6页
  • 会议地点 San Diego CA(US)
  • 作者单位

    Department of Optics and Photonics, National Central University, Taoyuan, Taiwan, 320 Graduate Institute of Electro-optical Engineering, Chang Gung University, Taoyuan, Taiwan, 333;

    Graduate Institute of Electro-optical Engineering, Chang Gung University, Taoyuan, Taiwan, 333 Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan, 112;

    Graduate Institute of Electro-optical Engineering, Chang Gung University, Taoyuan, Taiwan, 333 Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan, 112 Chang Gung Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan 333;

    Department of Optics and Photonics, National Central University, Taoyuan, Taiwan, 320;

    Department of Optics and Photonics, National Central University, Taoyuan, Taiwan, 320 Graduate Institute of Electro-optical Engineering, Chang Gung University, Taoyuan, Taiwan, 333 Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan, 112;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 TP212.3;
  • 关键词

    swine-origin influenza A; H1N1; surface plasmon; biosensor; heterodyne;

    机译:猪源性甲型流感; H1N1;表面等离激元生物传感器外差;

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