Differential mRNA Expression of Insulin-like Growth Factor-1 splice Variants in Patients With Idiopathic Pulmonary Fibrosis and Pulmonary Sarcoidosis

摘要

Insulin-like growth factor-1 (IGF-1) is a highly mitogenic polypep- tide detectable in human lung. Using competitive reverse tran- scriptase/polymerase chain reaction (RT-PCR), expression of four IGF-1 transcripts was examined in bronchoalveolar lavage cells (BALC) from normal subjects, idiopathic pulmonary fibrosis (IPF), stage L/LL (no fibrosis), and stage III/IV (confirmed fibrosis) pulmo- nary sarcoidosis patients, and fibroblast strains isolated from nor- mal and IPF lungs.