Interspecific Hybridization of Brassica campestris x B. oleracea Through Ovary Culture

摘要

Using three varieties of Brassica campestris, Hauarad (708), Maoshan-3 (714) and Youbai (715), as the maternal plants and one variety of Brassica oleracea Jingfeng-1 (6012) as paternal plants, crosses were made to produce interspecific hybrids through ovary culture techniques. The ovaries from the cross between B. campestris x B.oleracea (708 x 6012 and 714 x 6012) were cultured and. ovary culture was more effective in terms of obtained seeds when ovaries were cultured in vitro at 9 d after pollination (DAP) . While for the cross of 715 x 6012, it was better when ovaries in vitro cultured at 12 DAP. Among three cross combinations, the cross of 714 x 6012 showed the best response and 43 seeds per ovary were obtained. Among the media studied, the ovaries from the cross of 708 x 6012 cultured on MS media supplemented with 3.0 mg L~(-1) BA x 0.1 mg L~(-1) NAA showed better response, and its rate of seeds per ovary reached 44.0%. While the ovaries from the other two crosses (714 x 6012 and 715 x 6012) showed the best response when cultured on B5 media supplemented with 3.0 mg L~(-1) BA + 0.2 mg L~(-1) NAA, and the rates of seeds per ovary reached 72.0 and 60.0%, respectively. All seeds obtained from the three cross combinations were cultured on the MS media supplemented with 1.0 mg L~(-1) BA + 0.05 mg L~(-1) NAA, and the seeds from the cross of 715 x 6012 showed the best germination response and the percentage of germinations reached 66.7%. The regenerated plantlets were obtained from these seedlings after cultured on the MS media supplemented with 0.05 mg L~(-1) NAA. Cytological study showed that these regenerated plants were all true hybrids of B. campestris x B.oleracea.