Optimization of Different Medical and Edible Plant Formulation in Growth-Promoting Effects for L. Bulgaricus using Mixture Design

摘要

To improve the activity of probiotics and take advantage of abudant medical and edible plants in China, 18 kinds of medical and edible plants which were chosen to study growth-promoting effects for L. Bulgaricus by single factor, 8 kinds of plants that have significant growth-promoting effect through preliminary screening have obtained. On the basis of this, the different combinations which study growth-promoting effects to L. Bulgaricus using simplex-centroid experimental design and the viable number was chosen as detection index. The result shows that the medical and edible plants of obvious growth-promoting effects are Coix seed, Lotus seeds, Wheat germ, Chinese yam, Pueraria, Lilium, Mulberry, Jujube. The optimal combination according to simplex-centroid experimental design is Coix seed 5.507%, Lotus seeds 30.834%, Wheat germ 20.545%, Chinese yam 20.545%, Pueraria 4.739%, Lilium 15.259%, Mulberry 6.321%, Jujube 6.321%, the viable number is up to 3.89×10~8 cfu/mL.