沖縄島産マングースにおけるELISA法を用いた血清診断のための二次抗体の選定

摘要

感染症の検査として従来から用いられている病原体の培養を主とする検査法は，検査結果を得るまでに時間がかかるという欠点を有する・経済性と鋭敏性に優れるIgG-ELISA法は，培養法に比べて検査時間の短縮に貢献できるが，検査対象の動物種ごとに適切な二次抗体の選定が必要である．そこで今回ジャワマングースについて市販の11種類の二次抗体について検討した．その結果，抗－フェレットⅠ〆；とプロテインAの2種類において，ジャワマングースのIgG抗体との接合による発色反応が認められた．このうち，プロテインAはジャワマングース個体間において反応の強さの有意差が認められたが，抗－フェレットでは反応の強さはプロテインAより低いが，ジャワマングース個体間の差は少なく，二次抗体の希釈倍率に対して一定の変化を示した．以上のことから，ジャワマングースにおけるELISA法に最適な二次抗体は抗-フェレットIgGであると考えられた．%The small Asian mongoose (Herpestes javanicus) was introduced from India in 1910 to control rat and habu snake (Protobothrops flavoviridis) populations on Okinawajima island. It has been clarified that the mongoose possesses Leptospira and Salmonella. On human beast common infectious disease with these situations or the possibility in which the mongoose propagates, it is very important to investigate the infection status in prevention of epidemic. There is variously serodiagnostic method epidemiologically used at present, and it has the defect for economical efficiency and sensitivity. However, it is possible to comparatively little do in labor and time that the IgG-ELISA method is excellent for economical efficiency and both sensitivity and that it catches it in the experiment. In this experiment, secondary antibody which was optimum for doing the IgG-ELISA method using the serum of the mongoose for the mongoose was selected. Eleven kinds of secondary antibody marketed were used for the experiment. In-human, rabbit, rat, guinea pig, hamster, cat, mouse IgG, protein G, protein L that there was color reaction by the junction with IgG antibody of the mongoose in two types of anti-ferret IgG and protein A and that the hoop resists it, the reaction hardly could be accepted. However, there was the significance for OD value which became an index to the intensity of the reaction by the individual in protein A. And, the difference of the OD value between individuals of the result which the OD value is inferior to protein A showed the proportion of the change in which the OD value is fixed for the dilution degree of the secondary antibody small in anti-ferret IgG Therefore, available and optium secondary antibody to the mongoose seems to be anti-ferret IgG