Effect of single mismatches at 3′–end of primers on polymerase chain reaction

摘要

Objective and MethodTo investigate the effect of three different mismatches (G/T, G/A or G/G) at the 3′–end of a primer to amplify a 268 bp (base pair) region of the human β–globin gene using different annealing temperatures (45 to 65°C).