Strain-specific bioaccumulation and intracellular distribution of Cd2+ in bacteria isolated from the rhizosphere ectomycorrhizae and fruitbodies of ectomycorrhizal fungi

摘要

Bioaccumulation of Cd²⁺ in soil bacteria might represent an important route of metal transfer to associated mycorrhizal fungi and plants and may have potential as a tool to accelerate Cd²⁺ extraction in the bioremediation of contaminated soils. The present study examined the bioaccumulation of Cd²⁺ in 15 bacterial strains representing three phyla (Firmicutes, Proteobacteria, and Bacteroidetes) that were isolated from the rhizosphere, ectomycorrhizae, and fruitbody of ectomycorrhizal fungi. The strains Pseudomonas sp. IV-111-14, Variovorax sp. ML3-12, and Luteibacter sp. II-116-7 displayed the highest biomass productivity at the highest tested Cd²⁺ concentration (2 mM). Microscopic analysis of the cellular Cd distribution revealed intracellular accumulation by strains Massilia sp. III–116-18, Pseudomonas sp. IV-111-14, and Bacillus sp. ML1-2. The quantities of Cd measured in the interior of the cells ranged from 0.87 to 1.31 weight % Cd. Strains originating from the rhizosphere exhibited higher Cd²⁺ accumulation efficiencies than strains from ectomycorrhizal roots or fruitbodies. The high Cd tolerances of Pseudomonas sp. IV-111-16 and Bacillus sp. ML1-2 were attributed to the binding of Cd²⁺ as cadmium phosphate. Furthermore, silicate binding of Cd²⁺ by Bacillus sp. ML1-2 was observed. The tolerance of Massilia sp. III-116-18 to Cd stress was attributed to a simultaneous increase in K⁺ uptake in the presence of Cd²⁺ ions. We conclude that highly Cd-tolerant and Cd-accumulating bacterial strains from the genera Massilia sp., Pseudomonas sp., and Bacillus sp. might offer a suitable tool to improve the bioremediation efficiency of contaminated soils.Electronic supplementary materialThe online version of this article (doi:10.1007/s11356-014-3489-0) contains supplementary material, which is available to authorized users.