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An Assemblable, Multi-Angle Fluorescence and Ellipsometric Microscope

机译:可组装的多角度荧光椭圆偏振显微镜

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摘要

We introduce a multi-functional microscope for research laboratories that have significant cost and space limitations. The microscope pivots around the sample, operating in upright, inverted, side-on and oblique geometries. At these geometries it is able to perform bright-field, fluorescence and qualitative ellipsometric imaging. It is the first single instrument in the literature to be able to perform all of these functionalities. The system can be assembled by two undergraduate students from a provided manual in less than a day, from off-the-shelf and 3D printed components, which together cost approximately $16k at 2016 market prices. We include a highly specified assembly manual, a summary of design methodologies, and all associated 3D-printing files in hopes that the utility of the design outlives the current component market. This open design approach prepares readers to customize the instrument to specific needs and applications. We also discuss how to select household LEDs as low-cost light sources for fluorescence microscopy. We demonstrate the utility of the microscope in varied geometries and functionalities, with particular emphasis on studying hydrated, solid-supported lipid films and wet biological samples.
机译:我们为具有显着成本和空间限制的研究实验室推出了多功能显微镜。显微镜围绕样品旋转,以直立,倒置,侧面和倾斜的几何形状进行操作。在这些几何形状下,它可以执行明场,荧光和定性椭圆偏振成像。它是文献中第一个能够执行所有这些功能的仪器。该系统可由两名本科生在不到一天的时间内从提供的手册,现成的和3D打印的组件组装而成,按2016年的市场价格计算,总成本约为1.6万美元。我们提供了详细说明的组装手册,设计方法摘要以及所有相关的3D打印文件,以期使设计的实用性超过当前的组件市场。这种开放式设计方法使读者可以根据特定需求和应用定制仪器。我们还将讨论如何选择家用LED作为荧光显微镜的低成本光源。我们展示了显微镜在各种几何形状和功能中的实用性,特别着重于研究水合的,固体支持的脂质膜和湿生物样品。

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