Cell-Surface Marker Signatures for the Isolation of Neural Stem Cells Glia and Neurons Derived from Human Pluripotent Stem Cells

摘要

BackgroundNeural induction of human pluripotent stem cells often yields heterogeneous cell populations that can hamper quantitative and comparative analyses. There is a need for improved differentiation and enrichment procedures that generate highly pure populations of neural stem cells (NSC), glia and neurons. One way to address this problem is to identify cell-surface signatures that enable the isolation of these cell types from heterogeneous cell populations by fluorescence activated cell sorting (FACS).