2029. Comparison of Primers Amplifying Two Different Regions of the 16S Ribosomal RNA Gene for Microbiologic Diagnosis of Cardiovascular Implantable Electronic Device Infection

摘要

BackgroundBacterial cultures are negative in over 13% of cases of Cardiovascular Implantable Electronic Device (CIED) Infections. Broad-range PCR and Sanger sequencing could potentially establish a microbiologic diagnosis in these patients. We aim to evaluate and compare detection and sequencing performance of primers targeting two different hypervariable regions of the 16S ribosomal RNA (rRNA) gene on sonicate fluid from extracted CIEDs.