Phylogeny of interleukins: growth factors produced by leucocytes of the cyprinid fish Cyprinus carpio L.

摘要

Supernatants of phytohaemagglutinin (PHA)-activated pronephric leucocytes from carp (Cyprinus carpio L.) contain a lymphocyte growth factor which can induce a proliferative response of purified lymphoblasts but not freshly isolated leucocytes. The growth-promoting activity can be reduced by absorbing the supernatant with mitogen-activated blasts. In addition, increased incorporation of tritiated thymidine into PHA-activated blast cells is also induced by supernatants from two-way mixed leucocyte cultures. The data show that even at the evolutionary level of teleost fish, amplifying/regulatory leucocyte products exist. It is suggested that these factors play as important a role in the regulation of the immune response in fish as they do in mammals.