Potential role of MRN-100 an iron-based compound in upregulatingproduction of cytokine IL-10 in human dendritic cells to promote ananti-inflammatory response in vitro

摘要

The hydroferrate fluid MRN-100, an iron-based compound with potent antioxidant characteristics, was examined to identify its possible anti-inflammatory effects on human dendritic cells (DCs) in vitro. Human monocyte–derived DCs were treated with MRN-100 at two concentrations (50 and 100 μL/mL) for 24 h and then stimulated with or without lipopolysaccharides (LPS). The expression of DC maturation markers was assessed by flow cytometry and the production of cytokines was determined by enzyme-linked immunosorbent assay (ELISA). Functional assay was performed by co-culturing MRN-100-treated and untreated DCs with allogeneic naïve CD4+ T cells and assaying the T cells’ cytokine production. Results show that treatment with MRN-100 significantly upregulated the co-stimulatory molecules CD80 and CD86 and increased human leukocyte antigen-DR (HLA-DR) though not significantly. MRN-100 treatment also significantly increased the production of the anti-inflammatory cytokine interleukin (IL)-10. On the other hand, MRN-100 significantly induced the secretion of pro-inflammatory cytokines such as IL-6 only at high concentrations. Furthermore, DCs pretreated with MRN-100 and either stimulated or not with LPS were able to prime CD4+ T cells to secrete significant amountsof IL-10 while inhibiting the secretion of pro-inflammatory cytokine tumornecrosis factor (TNF)-α. These results indicate that MRN-100 is a powerfulanti-inflammatory agent that promotes the generation of an anti-inflammatoryimmune response in vitro. MRN-100 could be beneficial for treating patients withinflammatory diseases, including arthritis and type 1 diabetes, and itspotential benefits should be examined in clinical trials.