Vitamin C may impact the efficiency of radiation therapy (RT) in breast cancer. The effects of RT alone or in combination with vitamin C in SKBR3, MDA-MB-231, and MCF7 cells were compared using clonogenic assay, proliferation assay (MTT), cell cycle analysis, and Western blot. Vitamin C use was assessed in 1803 breast cancer patients 2002–2017 in relation to clinicopathological features and recurrences after RT. Vitamin C combined with RT resulted in non-significant increases in colony formation and minor differences in cell cycle arrest and expression of studied proteins, compared to RT alone. Lower vitamin C doses alone or in combination with RT, resulted in higher proliferation with MTT than higher vitamin C doses in a cell line-dependent manner. Vitamin C use was associated with lower histological grade and BMI but not recurrence risk in RT-treated patients (LogRank P = 0.54). Vitamin C impacted RT efficiency differently depending on breast cancer subtype and vitamin C concentration. Lower doses of vitamin C, achievable with oral administration, might increase breast cancer cell proliferation and decrease radiosensitivity. Despite vitamin C users having less aggressive tumors than non-users, the recurrence risk in RT-treated patients was similar in vitamin C users and non-users.
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机译：维生素 C 可能会影响乳腺癌放射治疗 （RT） 的效率。使用克隆形成试验、增殖试验 （MTT） 、细胞周期分析和 Western blot 比较 RT 单独或联合维生素 C 对 SKBR3 、 MDA-MB-231 和 MCF7 细胞的影响。在 1803 年至 2002 年期间，2017 名乳腺癌患者评估了维生素 C 的使用与临床病理特征和 RT 后复发的关系。与单独 RT 相比，维生素 C 联合 RT 导致集落形成没有显着增加，细胞周期停滞和研究蛋白质表达也有微小差异。在细胞系依赖性方式下，较低维生素 C 剂量单独或与 RT 联合使用导致 MTT 的增殖高于较高维生素 C 剂量。维生素 C 的使用与较低的组织学分级和 BMI 相关，但与 RT 治疗患者的复发风险无关 （LogRank P = 0.54）。维生素 C 对 RT 效率的影响因乳腺癌亚型和维生素 C 浓度而异。口服可达到较低剂量的维生素 C 可能会增加乳腺癌细胞增殖并降低放射敏感性。尽管维生素 C 使用者的肿瘤侵袭性低于非使用者，但 RT 治疗患者的复发风险在维生素 C 使用者和非使用者中相似。
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