Development of a Rapid Derivative Spectrophotometric Method for Simultaneous Determination of Acetaminophen Diphenhydramine and Pseudoephedrine in Tablets

摘要

A mixture of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride is used for the symptomatic treatment of common cold. In this study, a derivative spectrophotometric method based on zero-crossing technique was proposed for simultaneous determination of acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride. Determination of these drugs was performed using the ¹D value of acetaminophen at 281.5 nm, ²D value of diphenhydramine hydrochloride at 226.0 nm and ⁴D value of pseudoephedrine hydrochloride at 218.0 nm.The analysis method was linear over the range of 5-50, 0.25-4, and 0.5-5 µg/mL for acetaminophen, diphenhydramine hydrochloride and pseudoephedrine hydrochloride, respectively. The within-day and between-day CV and error values for all three compounds were within an acceptable range (CV<2.2% and error<3%). The developed method was used for simultaneous determination of these drugs in pharmaceutical dosage forms and no interference from excipients was observed.