Translocation in Bacillus subtilis: Irreversible Inactivation of Elongation Factor G and Viability of a Temperature-Sensitive Mutant

摘要

A temperature-sensitive mutant of Bacillus subtilis 168 lost its viability irreversibly when grown at temperatures higher than 50 C. It is suggested that this loss of viability is due to irreversible alteration of elongation factor G activity, which was shown in vitro by two different assay methods.