The power output in cycling is one of the most important factors for athletes and coaches. The cycling community has several commercial gears that can be used. One of the most used is the TACX Neo 2T (TN2T) smart trainer. The objective of this study was to investigate the metrological proprieties of the TN2T (accuracy and reliability), as well as its agreement with the Garmin Vector 3 (GV3) pedals at different power stages. The sample consisted of ten regional-level cyclists with a mean age of 45.6 ± 6.4 years, who regularly participated in regional and national competitions. Residual relative differences were found between the two devices. Both devices showed good reliability with coefficients of variation and intraclass correlation coefficients ranging from 0.03% to 0.15% and from 0.731 to 0.968, respectively. Independent samples t-test comparison between devices showed no significant differences in all power stages (p > 0.05). Bland–Altman plots showed that more than 80% of the plots were within the 95% confidence intervals in all power stages. The present data showed that there were non-significant differences between the two devices at power stages between 100 W and 270 W, with a strong agreement. Therefore, they can be used simultaneously.
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